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If the installer is of any size, it may take time depending on the speed of your Internet connection. Run the installer, follow the prompts to complete the installation.PROJECT SUMMARY/ABSTRACT HIV-1 infection remains a major cause of morbidity and mortality in the US despite highly active antiretroviral therapy (HAART). HIV-1 infection results in chronic inflammation, immune dysfunction, and neurocognitive impairment, among other pathologies, that limit the efficacy of HAART. HIV-1 infection leads to alterations in gene expression in both neurons and astrocytes, but the mechanisms and cellular consequences of these changes remain unclear. The overall objective of this proposal is to identify the cellular mechanisms of HIV-1-induced dysfunction in astrocytes and neurons, and to examine how HIV-1 alters gene expression in astrocytes and neurons and interferes with cellular functions. Preliminary data from our laboratory shows that HIV-1 infection in astrocytes leads to activation of the TAK1-NF?B-dependent pathway. TAK1 is a member of the TAK1/TAB1/TAB2 complex and is activated by the TLR pathway upon binding of viral RNA. Activated TAK1, in turn, initiates the IKK-NF?B pathway and subsequently NF?B, which is involved in the regulation of immune responses, inflammation, and cell survival. The proposed research will utilize a combination of molecular, cellular, and mouse modeling approaches to: 1) determine the role of TAK1 in HIV-1-induced astrocyte dysfunction and whether the TAK1 pathway is activated in astrocytes and neurons following HIV-1 infection, 2) determine the role of the TAK1 pathway in regulating HIV-1-induced activation of the NLRP3 inflammasome in astrocytes, 3) determine how HIV-1 infection alters neuronal survival through the TAK1-NF?B-dependent pathway, and 4) determine the role of TAK1 in modulating cellular functions in ast 8e68912320 Flash Decompiler Trillix 5.0.1060 Download Help you save time with your Mac. KeyMacro makes it easy to create keyboard shortcuts. Just double-click a key on your keyboard and start typing to define the keyboard shortcut that you want. The shortcut is created automatically based on what you type. Here are some examples of how KeyMacro can help you. Create a shortcut for an application. 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